Of note, we did not observe any significant change of CD8+ T cell activation in the tumor-draining lymph nodes (TdLN; see SI Appendix, Fig. S3B), suggesting that Pikfyve-loss in malignant cells affects quantity and functionality of CD8+ T cells in the tumor microenvironment, rather than CD8+ T cell priming in TdLN. This evidence concerns the gene CD8A and neoplasm.