While PIKfyve inhibition elevated MHC-I surface expression in wild-type cells, it did not further increase surface expression of MHC-I in the Atg5-null or Atg7-null cancer cells, confirming that PIKfyve inhibition upregulated MHC-I surface expression by impairing autophagy (Fig. 1G). This evidence concerns the gene PIKFYVE and cancer.