BNIP3 and infection: Fig 3A shows representative images of the 10 h time-point. As expected [49], quantification of red-only puncta in cells treated with the iron-chelator DFP, a known mitophagy inducer, and transfected with siScramble or siBNIP3, showed a clear increase when compared to mock-treated cells, with an average of 5.8 and 9.9 red-only puncta per cell, respectively (Fig 3B). However, no changes in the mitophagy flux were seen upon CHIKV infection at any of the assessed time-points, irrespective of BNIP3 expression, even when cells depleted for BNIP3 were more susceptible to infection (Fig 3C).