Briefly, flanking 5 μM serial sections were stained either with H&E for pathologist review to define regions of interest with viable tumor content or with monoclonal antibody-fluorescent dye conjugates of E-cadherin, vimentin, and β-catenin, along with 40,6-diamidino-2-phenylindole (DAPI). This evidence concerns the gene VIM and neoplasm.