OCLN and colorectal carcinoma: Indeed, among the 6 barrier-related genes tested, only heat treated CEC15 at multiplicity of infection (MOI) of 100:1 induced the expression of Muc2, while only heat treated EcN at MOI of 100:1 lowered the expression of Ocln. In addition, Ptsg2 expression, which in the colonic environment is highly associated with the promotion of colorectal carcinoma, was only induced by EcN (Fig. 6).