We then established stable EMX1 transcript-specific overexpression in two mesenchymal-like cell lines (SNU-398 and SNU-182) and two hepatoblast-like cell lines (Hep-G2 and Huh-7) as well as knockdown in SNU-182 and SNU-398 to assess the functions of EMX1 in HCC. This evidence concerns the gene EMX1 and hepatocellular carcinoma.