After analyzing BSP sequencing results, we noticed positive correlations between EMX1 gene body methylation and mRNA expression following R2 (r = 0.80, P < 0.01), R4 (r = 0.70, P = 0.01) and R3 (r = 0.52, P = 0.08) in tumor (Fig. 1E). This evidence concerns the gene EMX1 and neoplasm.