Reanalysis of a comprehensive dataset published by Wihelmi et al.21 that compared gene expression in islets from adult mice with obesity but not diabetic carrying a homozygous mutation in the leptin gene (Lepob, ob/ob, ND) to adult mice with obesity, hyperglycemia, and insulin resistance (New Zealand Obese, NZO, T2D) confirmed 1973 significant differential splicing events across 1,339 genes in diabetic conditions (Fig. S1Α). This evidence concerns the gene LEP and obesity due to melanocortin 4 receptor deficiency.