This AE model of mice was established by intranasally infusion with Spn strains (1 × 105 CFU) at 14th day after BLM administration, compared with those treated with controls (BLM or Spn alone), the AE mice showed more severe pulmonary fibrosis and collagen deposition when sacrificed on Day 17, and the morbidity and mortality of the mice showed a dose dependence of Spn [63]. The gene discussed is SPN; the disease is pulmonary fibrosis.