In this context, this study aimed to investigate the anti-inflammatory effects of E. cava and C. indicum complex extract (ED), which demonstrated anti-inflammatory effects in an OVA-induced asthma model, on MUC5AC inhibition in PMA-induced pulmonary epithelial cells and their impact on pulmonary inflammatory responses in a PM2.5-induced pulmonary inflammation mouse model. This evidence concerns the gene MUC5AC and asthma.