In addition, we validated our HFD obesity-NAFLD animal model by reporting significant increments in the rats’ final body and fat pad weights, fasting hyperglycemia, hyperlipidemia, hyperinsulinemia), higher HOMA-IR (a marker of IR), increases in the serum levels of ALT, AST, and GTT, and via the obvious degeneration and ballooning in the hepatocyte structure. The gene discussed is GPT; the disease is obesity due to melanocortin 4 receptor deficiency.