Another approach for obtaining organoids from HPV+ tumor tissue involves the implantation of tumor fragments in NOD/SCID/IL-2Rγ−/− mice to a <25% stable engraftment rate; after the xenograft reaches a volume of 1 cm3, it was passaged at least twice, removed, dissociated into individual cells, and cultured on ECM to form organoids. The gene discussed is IL2RG; the disease is neoplasm.