TNF and tuberculosis: Intracellular cytokine (IFN-γ, IL-2, and TNF-α) production by CD4+ and CD8+ T cells from Ugandan children with confirmed TB, unconfirmed TB, non-TB LRTI, and adults with confirmed TB, in response to five different mycobacterial antigens previously shown to be immunogenic in Mtb-infected adults [33], was assessed by flow cytometry (Table 1).