Surprisingly, atria-specificity was preserved upon induction of heart failure via pressure overload (transverse aortic constriction, TAC) after AAV9-mediated systemic gene transfer of a Cre-recombinase under control of the ANF promoter in Tomato reporter mice, a transgenic line enabling sensitive tracing of Cre expression using a red-green fluorescence reporter switch [33]. The gene discussed is NPPA; the disease is heart failure.