CD40LG and Lyme disease: CDC criteria for positive IgG immunoblots [27] require at least five of the following ten bands: 18-, 23-, 28-, 30-, 39-, 41-, 45-, 58-, 66-, and 93-kDa; only IgG immunoblots are advised for diagnosis more than 30 days after disease onset, although some investigators have suggested using IgM immunoblots to diagnose Lyme neuroborreliosis up to 6 weeks after disease onset [10].