INS and Alzheimer disease: However, more recently, GLUT3 translocation has been described again in the hippocampus of 3xTg-AD mice [66], but this report has some methodological problems such as: (1) no information regarding the insulin stimulus applied; (2) no information regarding the number of animals used for statistical analysis; (3) GAPDH was inappropriately used for normalization of the results in the plasma membrane (this protein is not bound in the PM); (4) insulin did not increase PM GLUT3 in control mice; and (5) GLUT3 content, actually, decreased in the PM of insulin-stimulated 3xTg-AD mice.