To control for cytotoxic effects of the CRISPR/Cas9-induced DNA damage response on the growth and survival parameters of the cells we targeted the third intron of troponin I3, cardiac type (TNNI3), which is not essential in B-ALL and MM cell lines (DepMap, accessed on 13 November 2022). The gene discussed is TNNI3; the disease is Miyoshi myopathy.