Earlier efforts of neurosphere generation date back to the early 1990s [143,144], and in the early 2000s, several studies demonstrated the successful generation of neurospheres from putative glioma stem cells (GSCs) cultured with neurotrophic growth factors like epidermal growth factor (EGF) and FGF2 [145,146,147,148]. This evidence concerns the gene EGF and glioma.