The subTME including subTME1 in BC, subTME2 in ESCA, subTME4 in PDAC, and subTME5 in RCC was enriched by stromal cells including Angiogenic_EC, ecm_myCAF, Macro_SPP1, and malignant epithelial cells. Here, SPP1 is linked to renal cell carcinoma.