With regard to NASH, in vitro studies have shown that the NLRP3 inflammosome can sense lipotoxicity-associated increases in intracellular levels of ceramides, cholesterol crystals, saturated fatty acid content, mtDNA levels, and ROS content, causing caspase-1 induction in macrophages and subsequently promoting IL-1β production [129,130,131,132]. This evidence concerns the gene CASP1 and metabolic dysfunction-associated steatohepatitis.