The expression of NOTCH1 and NOTCH3 mRNA and fold changes in monocultures of UT-SCC-42B, UT-SCC-24B, and CAFs, compared to 3D cocultures of different tumor cells with varying ratios to CAFs (3:1, 1:1, 1:3), was performed by qRT-PCR. This evidence concerns the gene NOTCH1 and neoplasm.