Following treatment with CCl4 thrice a week for 6 weeks, mice containing BMM Gpr65 knockout exhibited attenuated hepatic fibrosis, inflammation and injury than WT/BMT-WT controls, as demonstrated by HE staining, Sirius red staining, Masson’s trichrome staining, qRT-PCR, Western blotting, liver hydroxyproline content as well as serum ALT and AST level (Additional file 1: Fig. S6a-f). The gene discussed is GPR65; the disease is Hepatic fibrosis.