To confirm this, we performed HE staining, Sirius red staining, Masson’s trichrome staining and IHC for COL1α1 and α-SMA, and the results revealed that loss of Gpr65 significantly alleviated BDL-induced hepatic fibrosis (Fig. 2f; Additional file 1: Fig. S3e). The gene discussed is COL1A1; the disease is Hepatic fibrosis.