As shown in Fig. 7a–d, GPR65 inhibition significantly attenuated CCl4-induced hepatic fibrosis, as evidenced by HE staining, Sirius red staining, Masson’s trichrome staining, IHC, Western blotting and qRT-PCR for pro-fibrogenic genes, as well as liver hydroxyproline content. The gene discussed is GPR65; the disease is Hepatic fibrosis.