MYCN and central nervous system cancer: When we compared MYCN expression in human and mouse HGG-MYCN relative to other human gliomas or healthy mouse tissue of the OB and cerebellum and the SHH-MB mouse model, and performed gene set enrichment analysis (GSEA) for MYCN target genes, we found that MYCN itself as well as its target gene sets are significantly enriched in tumors of both species, human and mouse (Fig. 3e, f, two-sided Welch’s-t-test, human: p = 0.0002, 95% confidence interval = 2.001 to 4.320, murine= p = 0.0002, 95% confidence interval = 2.143 to 4.085).