Additional analysis of the FMI and PAD subsets (Supplementary Fig. S4a, b), as well as analysis of the FMI subset for co-occurrence of mutations in key CRC driver genes, with either individual PTEN mutational hotspots, or cumulatively for belonging to other hotspots, or non-hotspots, also revealed no significant differences in co-occurrence frequency in MT-L (Supplementary Fig. S4c, d) or MT-H (Supplementary Fig. S4e) tumors. The gene discussed is PTEN; the disease is colorectal carcinoma.