Linear regression analysis revealed that the percentage change from baseline in diameters of target lesions positively correlated with the Treg/Tef ratio (Supplementary Table 2, p = 0.0061, R2 = 0.68) but not Treg cells (p = 0.054, R2 = 0.43) or Tef percentage (p = 0.063, R2 = 0.41) (Fig. 6c, Supplementary Fig. 12b), directly linking the Treg/Tef ratio to the tumor control of T + A therapy. The gene discussed is TEF; the disease is neoplasm.