As illustrated of scRNA-seq analysis in Supplementary Fig. 2d and Fig. 2c, except for the CD8 T cell population, macrophages exhibited the highly enrichment among tumor-infiltrating immune cells (TIICs) in Hepa1-6 HCC tumors treated with LR-DPVB compared to the CON group. This evidence concerns the gene CD8A and hepatocellular carcinoma.