Additionally, Kaplan‒Meier Plotter analysis (https://kmplot.com/analysis/) showed that higher coexpression of TCF-1, PD-1 and CD8 was significantly related to better survival of HCC patients, while coexpression of TCF-1, PD-1 or expression of TCF-1 alone did not show a correlation with the prognosis of HCC patients (Supplementary Fig. 12a). This evidence concerns the gene PDCD1 and hepatocellular carcinoma.