MT-ND4 and neoplasm: To assess the ability for in vivo imaging of mtDNA mutations of InCasor, two cell lines derived from different types of tumors (HepG2 and MDA-MB-231) were used to construct tumor-bearing mouse models, since their mtDNA sequences were confirmed and the corresponding InCasor probes targeting different mtDNA mutation (ND4 and ND5) have been designed and tested in live cells (Fig. 4).