In addition, 11 biomarkers were associated with the risk of invasive breast cancer, among which the biomarkers with the smallest Ptrend included testosterone (HR for the highest quartile = 1.47, 95 % CI = 1.38–1.56), neutrophil count (HR for the highest quartile = 1.16, 95 % CI = 1.09–1.23) and IGF-1 (HR for the highest quartile = 1.17, 95 % CI = 1.10–1.25) (Fig. 2, Supplementary Table 6). This evidence concerns the gene IGF1 and invasive breast carcinoma.