When we looked across a range of effector-to-target ratios, the presence of DGKi during coculture could elicit cytotoxicity and IFN-γ production even when tumor cells outnumbered T cells by 5:1 or 10:1, ratios at which no cytotoxicity was detected from vehicle cultured cells or cells that had only seen DGKi at the time of priming (Fig. 5, E and F). This evidence concerns the gene DGKI and neoplasm.