In a meta-analysis of probiotic supplementation in pregnant women for prevention of GDM, 10 randomized controlled trials were included. A correlation was found between probiotic use and fasting serum insulin and insulin resistance. No significant correlation was seen between probiotic use and lipid levels in pregnant women with GDH. For healthy pregnant women, probiotics were negatively associated with fasting serum insulin. No correlations were found between probiotic use in fasting plasma glucose. This evidence concerns the gene INS and Insulin resistance.