Out of the 12 cryobiopsies conducted for lung malignancies, nine NSCLC (eight adenocarcinomas and one large cell neuroendocrine carcinoma) were valid for RT-PCR and PD-L1 IHC testing, leaving the EBUS-TBNA material for IHC determinations (p40 and TTF1). This evidence concerns the gene CD274 and adenocarcinoma.