The induction of caspase-1-mediated pyroptosis in non-small cell lung cancer (NSCLC) by Polyphyllin VI (PPVI) was seen via the signaling axis of ROS, nuclear factor kappa B (NF-κB), nod-like receptor family pyrin domain containing 3 (NLRP3), and gasdermin D (GSDMD). This evidence concerns the gene NFKB1 and non-small cell lung carcinoma.