Next, NASH mouse scRNA‐seq data (GSE166504) were used to confirm the regulatory steps from AMPK to JNK/c‐Jun in hepatocytes using SCENIC pipeline for dissecting TF regulatory network from normal tissues (Chow group) to liver‐injured tissues (15, 30 and 34 weeks of NASH). The gene discussed is JUN; the disease is metabolic dysfunction-associated steatohepatitis.