Increasing evidence suggests that the ECM serves as a niche for CSCs.[35] COL1, an important part of the extracellular matrix, has been linked to tumor growth and metastasis.[36] Furthermore, COL1 plays an important role as a scaffold of the CD133‐positive GSCs.[37] Supporting this notion, COL I promotes the expansion of CSCs.[38] Our findings illuminate the significance and mechanism of the interaction between CD133 and COL1 in the extracellular matrix microenvironment of GSCs. The gene discussed is PROM1; the disease is neoplasm.