Combined, these data indicate that neither fractionated radiation nor KCa3.1 targeting with TRAM-34 nor their combination, induced pronounced changes in the abundance of Iba1+ or CD68+ reactive microglia and CD3+, CD8+ cytotoxic or FoxP3+ regulatory T cells in the SMA-560 VM/Dk glioma model. Here, FOXP3 is linked to central nervous system cancer.