To further confirm that NOD1 was required within ECs to induce HE-like fate, we performed an independent experiment (see Methods for details) in which NOD1 was chemically inhibited by Nodinitib-1 at day 4, or days 4 + 6 of hematopoietic differentiation, and the percentage of CD34+ ECs analyzed at day 9 (Fig. 8d). The gene discussed is NOD1; the disease is hereditary elliptocytosis.