To further assess the roles of adipocyte SLC35D3 in the regulation of obesity and adipose inflammation, in “reverse” experiments, we generated adipose-specific Slc35d3-knock-in (SAKI) mice by crossbreeding Slc35d3-WT mice carrying the LSL-Slc35d3 transgene locus with adiponectin-derived Cre mice. This evidence concerns the gene ADIPOQ and obesity due to melanocortin 4 receptor deficiency.