In addition, ALOX5AP DNA methylation also affected gene expression levels, as we showed by targeted bisulfite sequencing that ALOX5AP gene methylation levels were significantly lower in AML patients compared to healthy controls, this was also validated by comparison of the Diseasemeth database and independent cohorts of GSE63409 normal and AML patients with ALOX5AP methylation levels. The gene discussed is ALOX5AP; the disease is acute myeloid leukemia.