CRISPR/Cas9 mediated Wnt1 gene KO in the HCT116 cell line reduced Wnt1 protein expression, which in turn promoted the release of inflammatory cytokines such as IL-8, IL-6, and GM-CSF and protected cells from Salmonella invasion and reduced tumor cell migration and invasion ability in vitro. This evidence concerns the gene WNT1 and neoplasm.