Furthermore, RNASET2 content in the seminal plasma detected by ELISA also revealed that RNASET2 expression significantly increased in the seminal plasma from astheno-teratozoospermia individuals (24.43 ± 4.39 μg/mL, n = 63) compared with that from healthy semen specimens (17.07 ± 4.48 μg/mL, n = 44, P < 0.05, Additional file 2: Figure S6F). Here, RNASET2 is linked to Abnormal sperm morphology.