As expected, the protein expression of BCAT2 and PP2Cm were higher in NAF1L2-deficient or SIRT3-activated CF than those of control cells in response to HG stimuli (Fig. 6H, I and Additional file 2: Fig. S2G), indicating that the NAF1L2/SIRT3 pathway was involved in hyperglycemia-induced dysfunction of BCAA catabolism. Here, PPM1K is linked to Hyperglycemia.