The PD-L1 gene level was higher in metastatic (1.39 ± 0.11) compared to non-metastatic (0.7 ± 0.05) osteosarcoma tumors (P < 0.0001), also metastatic (1.27 ± 0.05) compared to non-metastatic (0.51 ± 0.05) Ewing sarcoma tumors (P < 0.0001) (Fig. 1E). Here, CD274 is linked to osteosarcoma.