Additionally, the ROC curve analysis was applied to evaluate whether the PD-L1 gene and protein and TGF- β and IFN-γ levels can discriminate different patient groups and tumor subtypes and our data showed that the abovementioned factors could distinguish patients with bone tumors and healthy individuals, also malignant and non-cancerous tissues, GCT and non-cancerous tissues and malignant and GCT, significantly. Here, IFNG is linked to bone neoplasm.