The DNA mutations used for mapping occurred statistically in 460 (~ 89.7%) LUAD patients and 330 (~ 66.3%) LUSC patients according to the data, and the waterfall plot shows that the overall level of DNA alterations in the PBK gene in lung cancer is about 5% (Fig. 3A, B, D), and we found that the predominant PBK mutation type in lung cancer is Missense_Mutation (Fig. 3C). The gene discussed is PBK; the disease is lung carcinoma.