Moreover, inhibition of BRD4 either by shRNA or by (+)-JQ1 (10 μm) made cells more tolerant to erastin, which was manifested as an overall cell survival advantage, reduced MDA, lipid ROS and Fe2+ contents, and decreased mitochondrial damage rates (Fig. 1B–F), demonstrating the critical roles of BRD4 in erastin- ferrop of osteosarcoma cells. The gene discussed is BRD4; the disease is osteosarcoma.