In this study, we found that BRD4-SRPK2-SRSF2 constructed a processing platform of pre-mACSL3 splicing to ensure the expression abundance of ACSL3 in osteosarcoma cells, thus playing important roles in arachidonic acid synthesis based on its AMP-binding domain and subsequently impacting susceptibility to erastin-ferrop. The gene discussed is SRSF2; the disease is osteosarcoma.