The percentage of Florida men aged 40 years or older who reported receiving a PSA test in the past 2 years decreased 28.9%, from 51.2% (95% CI, 48.1%–54.4%) in 2012 to 36.4% (95% CI, 33.0%–39.7%) in 2020, representing 503,443 fewer Floridians screened for prostate cancer. The gene discussed is KLK3; the disease is Familial prostate cancer.