Relative to differences in prostate cancer screening prevalence between individual years within the period, the percentage of Florida male residents who received a PSA test in the past 2 years declined from 51.2% in 2012 to 49.7% (95% CI, 47.3%–52.2%) in 2014, a 2.9% decrease; and from 49.7% in 2014 to 46.2% (95% CI, 44.2%–48.2%) in 2016, a 7.0% decrease. This evidence concerns the gene KLK3 and prostate cancer.