PK15 cells were infected with PRV (0.5 MOI) for 4, 8, 12, 18, 24, and 36 hours, the infected samples were subjected to western blot analysis, and the results showed that BRAF, RPS6KA1, and STMN1 were all significantly upregulated during the infection of PRV, which demonstrated the reliability of our phosphoproteome (Fig. 5A). The gene discussed is RPS6KA1; the disease is infection.