Furthermore, ANOVA revealed that the number of platform crossings significantly differed among the groups (F(3, 31) = 25.706, p < .001), with the BD + Pin1 group crossing the platform location significantly more times than the DB and DB + 0 groups (p < .01 and p < .001, respectively, Figure 2c), suggesting that overexpression of Pin1 can alleviate diabetic encephalopathy. This evidence concerns the gene PIN1 and diabetic encephalopathy.