For subsequent analyses, we selected a LN-229 glioblastoma clone with no indel at the POLE sgRNA on-target site (clone 2, POLE WT) and the only LN-229 glioblastoma clone devoid of WT POLE alleles (clone 17, POLE KO) shown to exclusively harbor the POLE:c.84_85del p.(V29Ffs*14) frameshift variant at the sgRNA on-target site (Fig. 5b) and not to express full-length POLE by Western blot analysis (Fig. 5c). The gene discussed is POLE; the disease is glioblastoma.