EA at ST36 significantly decreased visceral pain and colon 5-HT3 receptor levels (Chu et al. 2011), and EA at ST25 and ST37 significantly decreased the colorectal distension (CRD)-induced abdominal withdrawal reflex (AWR), number of mucosal mast cells, expression of corticotropin-releasing hormone (CRH) in the hypothalamus, and expression of Substance P and its receptor in the colon of rats with IBS (Ma et al. 2009; Wu et al. 2008). This evidence concerns the gene CRH and irritable bowel syndrome.