By annotating candidate genes within a 1 Mb region surrounding the significant SNPs, a total of 11 candidate genes were identified on SSC5 and 13, including KRT71, KRT1, KRT4, ITGB7, CSAD, RARG, SP7, PFKL, TRPM2, SUMO3, and TSPEAR. These findings shed light on the genetic underpinnings of ACC in pigs, contribute to the understanding and treatment of ACC and provide a foundation for future studies elucidating the underlying molecular mechanisms. This evidence concerns the gene PFKL and adrenal cortex carcinoma.