We validated these findings by qRT-PCR and Western blotting of cell lysates of GC cells and ELISA of cellular medium (CM) of GC cells, Intriguingly, the mRNA and protein level of PDGF BB and TGF β1 were upregulated by HOXA11 in GC cells and similar findings were seen in CM of GC cells by ELISA assay (Fig. 5D–I), knockdown of HOXA11 in MGC803 cells exhibited an opposite effect (Fig. S2a–f). The gene discussed is HOXA11; the disease is gastric cancer.