Interestingly, similar findings were seen with immunofluorescence, western blotting, and qRT-PCR analysis of Egr1 expression in HMrSV5 cells (Fig. 6B–F), TGF β1/Smad signaling pathway activation had been confirmed to participate into the pathogenesis of peritoneal fibrosis [22, 23], western blotting analysis revealed that exogenous PDGF BB and TGF β1 induced the phosphorylation of Smad3 in HMrSV5 cells and addition of anti-human PDGF BB, TGF β1 neutralizing antibodies or BIBF could reverse this tendency (Fig. 6C). The gene discussed is SMAD3; the disease is Peritoneal Fibrosis.