When added to human vascular endothelium HUVEC cells (Fig. 1A, B), murine RAW 264.7 macrophages (Fig. 1C, D), rat cardiomyoblasts H9c2 cells (Fig. 2A, B), or human osteosarcoma U2OS cells (Fig. S1), stably expressing green fluorescent protein (GFP) fused with microtubule-associated protein 1 light chain 3 alpha/beta MAP1LC3A/B (best known as LC3), 3,4-dimethoxychalcone (3,4-DC) significantly induced the aggregation of LC3 across cell lines in cytoplasmic dots. The gene discussed is MAP1LC3A; the disease is osteosarcoma.