ESR1 and cancer: We selected 0.6–1.3-Mb domains around four ER-regulated genes: the estrogen receptor gene (ESR1) located on chromosome 6, the growth regulation by estrogen in breast cancer 1 gene (GREB1) located on chromosome 2, the cyclin D1 gene (CCND1) and the PGR on chromosome 11, and determined their 3D conformation using 5C (Dostie et al, 2006) in MCF7 and MDA-MB-231 cell lines (Fig 1B).